FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l02_n01_N_Light114.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l02_n01_N_Light114.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3969129
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGG35400.091.1071246
GTATAGG56450.090.706966
TAGCGCC1050.090.474579
CCGCGAT1000.090.298431
CTATACG31150.089.6613166
CTATAGC37100.088.852866
TCTATAC47300.088.6751255
GGTATAA87450.088.5923845
GGTATAC39650.088.532655
CTATACC36700.088.39766
CATATAG45600.088.230925
CTATACA70350.088.1114356
CCTATAC38500.088.092675
GGTATAG56950.087.9956055
GTATAGC40600.087.978726
CCTATAA80750.087.9429245
CCTATAG29900.087.852925
GCTATAA87100.087.748595
GGCAGGT1300.087.739371
CCTAGCT650.087.739371
Produced by FastQC (version 0.11.5)