FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l02_n01_N_Light113.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l02_n01_N_Light113.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6071198
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGGCTAAATACGGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGTA103600.1706417744899771No Hit
CATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGA95790.15777775654821338No Hit
CGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAAG83020.13674401658453572No Hit
GGGCGGTAAATTCCGTCCAAGGCTAAATACGGGCGAGAGACCGATAGCGA69300.11414551131424144No Hit
GCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAA63590.10474044826078807No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG164500.092.345355
CTATAGG88750.090.1277166
CTATACG68250.090.056746
GTATAGC77650.089.7373666
GTATAGG178450.089.727766
CGTATAC55650.089.1123355
CGTATAG88350.088.388915
TATAGGG198500.087.6757667
GGTATAA136600.087.6299065
TTATACG103950.087.275736
TATAGGC101350.087.171367
GCTATAG95400.087.0353245
AGTATAG108750.086.833965
GTATAGT153500.086.643166
GATATAG115900.086.313255
TATACCG77300.086.211027
CCTATAC40950.086.1861345
TTATAGC83750.086.0937356
AGTATAC58000.085.993145
GTTATAG171550.085.8921665
Produced by FastQC (version 0.11.5)