FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l02_n01_N_Light112.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l02_n01_N_Light112.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3776640
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGGCTAAATACGGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGTA53680.14213692594475513No Hit
CAACCATAAACGATGCCGACCAGGGATCAGCGGATGTTGCTTATAGGACT47240.12508473140145737No Hit
CATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGA44450.11769721233689204No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG99900.092.146485
CTATACG38500.091.296846
CGTATAC33800.089.940185
GTATAGG106000.089.710436
CTATAGG50550.089.548116
CGTATAG54000.088.314175
GTATAGC44600.087.5430456
TATACCG55100.087.0671847
GGTATAA84150.086.9839255
TATAGGG112600.086.940937
GCTATAG57550.086.828225
TATAGGC57950.086.7195367
GTATAGT96000.086.636246
CCTATAC26500.086.395615
CCTATAG32300.086.322915
AGTATAG62300.086.078835
GGGTATA114400.085.158914
TTATACG63800.085.096226
TATACGG84650.085.0661857
GTATACG64250.084.943796
Produced by FastQC (version 0.11.5)