FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l02_n01_N_Light111.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l02_n01_N_Light111.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4736285
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGGCTAAATACGGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGTA116500.2459733736462227No Hit
CGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAAG105400.22253728396834227No Hit
CATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGA82830.17488390162331868No Hit
GCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAA80040.168993208812392No Hit
GGGCGGTAAATTCCGTCCAAGGCTAAATACGGGCGAGAGACCGATAGCGA73110.15436148795944502No Hit
GTTGCTTATAGGACTCCGCTGGCACCTTATGAGAAATCAAAGTTTTTGGG50760.10717260468911816No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG54950.092.406246
GGTATAG126150.091.68895
CTATAGG70000.090.249516
GTATAGG139550.090.23426
GTATAGC59050.090.17316
CGTATAC44000.089.496685
GCTATAG69300.089.45045
TTATACG88450.089.360756
TTATAGC70200.088.909786
CGTATAG71150.088.326295
GTATACG94400.088.256956
GGTATAA106450.088.085925
GGTATAC71150.087.8589555
GTATAGT112050.087.665766
GATATAG81500.087.6004645
AGTATAG78700.087.397485
GTTATAG126650.087.1637655
TATAGGG156650.087.145137
TATAGGC83450.087.143617
TATAGCG116400.086.877927
Produced by FastQC (version 0.11.5)