FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l02_n01_N_Light110.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l02_n01_N_Light110.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3309647
Sequences flagged as poor quality0
Sequence length101
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG89350.088.3533865
GTATAGG87800.088.0737846
CGTATAG45450.087.473885
CTATAGG42900.086.915886
TATAGGG91200.086.769467
CTATACG31400.086.52736
GTATAGC40300.086.3944246
TATAGCG47550.084.909297
AGTATAG56350.084.293265
GGTATAA77800.084.070015
GGGTATA98550.083.768344
TTATACG47700.083.746066
GTATAGT85850.083.490246
CCTATAG27500.083.080515
GGTATAC43000.082.9585
TATAGGC47300.082.747117
TTATAGC48350.082.3254856
TATAGGT85600.082.180367
GCTATAG48300.082.017345
GCGTATA51650.081.939664
Produced by FastQC (version 0.11.5)