FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l02_n01_N_Light109.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l02_n01_N_Light109.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3822422
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGGCTAAATACGGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGTA85610.22396794493125038No Hit
GGGCGGTAAATTCCGTCCAAGGCTAAATACGGGCGAGAGACCGATAGCGA58360.15267806641966797No Hit
CAACCATAAACGATGCCGACCAGGGATCAGCGGATGTTGCTTATAGGACT41160.10768041833162324No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGCC450.094.997919
CGCGCTA3200.093.5564042
GGTATAG93850.091.1020055
CGTATAC32700.091.077165
GTATACG67150.090.965986
GGTATAC55450.090.716065
CCTATAC28900.090.397125
CTATACG54850.090.148426
CTATAGG64050.089.806766
AGCGCCT850.089.4507451
GTATAGG106200.089.362446
GATATAC45950.088.4866945
GTATAGC50900.088.1856846
TTATACG67600.088.182236
TATAGGT95000.087.548077
AGTATAG66900.087.402065
TATAAGG77500.087.030347
GCTATAG53750.086.780625
GTTATAG102800.086.68215
CCTATAG32650.086.124815
Produced by FastQC (version 0.11.5)