FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l02_n01_N_Light108.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l02_n01_N_Light108.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8409090
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG172750.090.080665
CTATAGG96300.089.129626
CCTATAC64100.089.074245
CGTATAG100800.088.829365
CTATACG70450.088.3917856
GTATAGG174200.088.29156
GTATAGC97050.087.461976
TATAGGG172100.086.994227
CCTATAG64250.086.5004655
GGTATAA185150.086.4849855
TATAGCG84500.086.341497
AGTATAG125900.085.230735
TATAGGC101250.085.005727
GCTATAC93600.084.903545
CGCTATA101600.084.763684
GGTATAC104100.084.553275
GCGTATA133950.084.044894
TTATAGC111150.083.9736946
CTATACC70150.083.6237266
GATATAG144800.083.520975
Produced by FastQC (version 0.11.5)