FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l02_n01_N_Light107.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l02_n01_N_Light107.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1542441
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGA27090.17563070483733254No Hit
CAACCATAAACGATGCCGACCAGGGATCAGCGGATGTTGCTTATAGGACT17640.11436417989407698No Hit
CAAGGCTAAATACGGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGTA16030.10392617934818901No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG16300.092.376796
GGTATAG37750.090.595535
GGTATAC22150.088.137225
TTATACG26550.088.0221256
CCTATAG12800.087.948745
GTATAGC18550.087.573646
GCTATAG23450.087.302295
GTTATAG40800.087.082865
CTATAGG23800.087.016336
CCTATAC12250.086.8566745
TATAGGG46900.086.086957
AGTATAG31000.085.9592065
GGTATAA34500.085.9125755
GCTATAC17150.085.85965
AGTATAC15050.085.846715
GTATAGT38150.085.7859046
CGTATAC13350.085.036995
TATAGTG36700.085.03367
CTATAGC16650.085.014566
GTATAGG41250.084.866216
Produced by FastQC (version 0.11.5)