FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l02_n01_N_Light106.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l02_n01_N_Light106.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3209022
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGA83800.2611387519312737No Hit
CAAGGCTAAATACGGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGTA58540.1824231806450688No Hit
GGGCGGTAAATTCCGTCCAAGGCTAAATACGGGCGAGAGACCGATAGCGA55300.1723266465608525No Hit
CGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAAG50770.1582101961282908No Hit
CAACCATAAACGATGCCGACCAGGGATCAGCGGATGTTGCTTATAGGACT46150.14381328641561197No Hit
GCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAA36130.11258881989590598No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32910.10255461009616013No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG79900.091.19885
CTATACG42500.091.084696
GTATAGC41700.090.895756
CTATAGG48650.090.505216
TTATAGC46700.089.198886
GTATAGG90000.089.085436
AGTATAG55400.088.4872065
TTATACG56700.088.37846
CGTATAC32650.088.020295
GTATACG65750.087.9889766
GGTATAA70400.087.716495
GCTATAG50950.087.545135
CGTATAG47700.087.534875
TATAGGC55550.087.471857
GTATAGT74750.087.307656
GGTATAC45700.087.2085
TATAGGG107550.087.179327
CCTATAC25650.087.0404365
GCTATAC37500.087.02345
GTTATAG83700.086.944855
Produced by FastQC (version 0.11.5)