FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l02_n01_N_Light105.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l02_n01_N_Light105.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3594073
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGA73510.20453118231043166No Hit
CAAGGCTAAATACGGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGTA57120.1589283244942437No Hit
CAACCATAAACGATGCCGACCAGGGATCAGCGGATGTTGCTTATAGGACT51700.14384794076247198No Hit
GGGCGGTAAATTCCGTCCAAGGCTAAATACGGGCGAGAGACCGATAGCGA46930.13057609013506405No Hit
CGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAAG43970.12234030861365365No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG87600.091.421985
CTATAGG51000.090.8083046
CTATACG43800.090.444696
GTATAGG95550.089.481446
GTATAGC47400.089.287476
CGTATAG50950.088.754415
CCTATAC27350.088.401095
GTATACG66450.087.994236
AGTATAG59550.087.981415
CGTATAC33300.087.868585
GGTATAC50950.087.728885
TATACCG53700.087.5693367
CCTATAG28950.087.289145
TATAGGG115000.087.1103747
AGTATAC34650.086.912695
TTATACG60950.086.816576
CTATACC31150.086.765156
TTATAGC57900.086.713666
GATATAG67600.086.498495
GTTATAG93200.086.387515
Produced by FastQC (version 0.11.5)