FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l02_n01_N_Light104.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l02_n01_N_Light104.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4673054
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGGCTAAATACGGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGTA85910.18384123102365177No Hit
CATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGA71940.15394643417345488No Hit
CAACCATAAACGATGCCGACCAGGGATCAGCGGATGTTGCTTATAGGACT56860.12167631702950576No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG56800.090.900356
GGTATAG92750.090.799595
GTATAGG107250.088.797656
CGTATAC44750.088.6303565
GTATAGC59300.088.269646
TTATACG77950.087.9295046
CGTATAG62400.087.463025
CCTATAG43650.087.381675
TATAGCG103900.087.317697
CTATAGG65850.087.2798546
GCTATAG70800.086.8140645
TATAGGC73900.086.4494557
CCTATAC38600.086.38515
TATACCG63850.086.368577
AGTATAG77150.086.3179555
TTATAGC73450.086.0737466
GGTATAA92400.085.4374165
GCTATAC55400.085.310485
TATAGGG123700.085.167967
TATACGG115450.085.164937
Produced by FastQC (version 0.11.5)