FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l02_n01_N_Light103.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l02_n01_N_Light103.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4979216
Sequences flagged as poor quality0
Sequence length101
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAACGATGCCGACCAGGGATCAGCGGATGTTGCTTATAGGACT119960.24092146233463263No Hit
GTTGCTTATAGGACTCCGCTGGCACCTTATGAGAAATCAAAGTTTTTGGG76290.1532168919765682No Hit
CAAGGCTAAATACGGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGTA71540.14367723754101047No Hit
CGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAAG68280.13713002207576455No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG99800.090.004375
CTATACG52200.089.720496
CGTATAC41000.089.093325
CTATAGG64750.088.249276
GTATAGC58600.087.6220256
TTATAGC81150.087.213356
CCTATAC32350.086.926085
TTATACG79450.086.7478566
TATAGGG131600.086.480097
GTATAGG115650.086.2088856
CTATACC40300.086.158396
CGTATAG60800.086.017425
GCTATAG70900.085.756375
TATACCG67400.085.695677
TATAGGC77400.085.547447
CCTATAG37450.085.48915
AGTATAG76400.085.054135
TATAGCG116600.084.6917657
GGTATAA95050.084.557255
GTATAGT113900.084.405746
Produced by FastQC (version 0.11.5)