FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l02_n01_N_Light102.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l02_n01_N_Light102.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5125360
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGGCTAAATACGGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGTA111810.2181505299139963No Hit
CATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGA100730.19653253625111214No Hit
CAACCATAAACGATGCCGACCAGGGATCAGCGGATGTTGCTTATAGGACT94080.18355783788846053No Hit
CGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAAG60470.11798195638940484No Hit
GGGCGGTAAATTCCGTCCAAGGCTAAATACGGGCGAGAGACCGATAGCGA59240.11558212496292943No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG127500.091.538315
CTATACG63600.091.264286
CTATAGG75400.090.210776
GTATAGG132350.089.471576
CGTATAC50400.089.253935
GTATAGC66700.088.945376
TATAGGC84000.088.6644067
TTATACG92400.088.624146
GCTATAG82400.088.3736655
CCTATAC37550.087.413015
GGTATAC74750.087.313915
TATAGGG159900.087.244497
CGTATAG69800.087.176955
TTATAGC79950.087.156236
AGTATAG83950.087.024915
GATATAG91700.086.870375
GTATAGT115300.086.553276
TATACCG76550.086.496817
GGTATAA110500.086.2765965
GTTATAG127600.086.180365
Produced by FastQC (version 0.11.5)