FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l02_n01_N_Light101.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l02_n01_N_Light101.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3716446
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGGCTAAATACGGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGTA55540.14944385038824726No Hit
CATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGA52930.14242101190223133No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG85050.090.5347445
CTATACG42100.089.696216
GTATAGC44400.088.901096
CGTATAG48250.088.800585
GTATAGG93150.088.0640266
CTATAGG52200.087.719396
TATAGGC60350.087.285747
GGTATAA78700.086.432065
TATAGGG107450.086.246197
CCTATAC28200.086.243715
GCTATAG57000.085.919245
CCTATAG29700.085.8864065
CGCTATA36000.085.106714
TATACGG87600.085.021947
CGTATAC35250.084.357135
TTATAGC57800.084.233546
TTATACG63750.084.120696
AGTATAG61700.084.070585
GTATAGT86550.083.9679266
GGTATAC47400.083.778885
Produced by FastQC (version 0.11.5)