FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l02_n01_N_Light100.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l02_n01_N_Light100.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4165911
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGGCTAAATACGGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGTA82050.19695571988935914No Hit
CATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGA76630.18394536033054953No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG104350.091.0414665
CTATACG45950.090.863496
CTATAGG60200.090.343116
GTATAGC49000.088.8913046
GTATAGG106050.088.593466
GCTATAC48850.088.195095
CGTATAG50900.087.3493655
GGTATAA89650.087.318975
CGTATAC41550.087.2278445
TTATAGC60950.086.893486
CCTATAG32700.086.576535
CCTATAC30100.086.480015
TATAGGG119850.086.477437
CTATACC32850.086.033516
GGTATAC56650.085.610595
TTATACG72350.085.347516
AGTATAG71050.085.30775
GCTATAG64850.085.186625
TATAGCG82050.085.0411767
TATAGGT90850.084.803147
Produced by FastQC (version 0.11.5)