FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l02_n01_N_Light099.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l02_n01_N_Light099.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3599979
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGA92340.25650149625872815No Hit
CAAGGCTAAATACGGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGTA67570.18769553933509056No Hit
CGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAAG44700.12416739097644736No Hit
GGGCGGTAAATTCCGTCCAAGGCTAAATACGGGCGAGAGACCGATAGCGA41100.11416733264277375No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA37050.10291726701739093No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG82900.091.274585
CTATAGG49650.090.692556
CTATACG39300.090.6465456
CCTATAC25750.090.3873145
GTATAGG87800.088.722116
CGTATAC35200.088.2518165
GTATAGC41350.088.2202456
CGTATAG46150.087.485455
TTATACG64600.087.350726
TATAGGC57100.087.3445367
TTATAGC53500.087.007136
TATAGGG106450.086.7427447
GGTATAC47600.086.716445
GTTATAG93800.086.137065
GCTATAG56250.086.04795
GCTATAC42500.085.946075
TATACCG46850.085.264647
CTATACC27800.085.258616
AGTATAG56900.084.897975
CCTATAG29850.084.8147665
Produced by FastQC (version 0.11.5)