FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l02_n01_N_Light098.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l02_n01_N_Light098.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3647770
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGA61970.1698846144356689No Hit
CAAGGCTAAATACGGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGTA57390.1573289982646932No Hit
GGGCGGTAAATTCCGTCCAAGGCTAAATACGGGCGAGAGACCGATAGCGA40490.11099932287397506No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG99900.090.772995
CTATAGG54250.090.445516
GTATAGG103850.089.647066
CTATACG39100.089.531786
CGTATAC35350.089.092345
CGTATAG50500.088.984835
TATAGGG117300.088.8016747
GTATAGC45300.087.973176
TTATACG62300.087.06926
AGTATAG59550.086.6291965
CCTATAG31100.086.6041955
TATAGGC57200.086.5271157
TATACCG39200.086.515247
TTATAGC52400.086.1156
GGTATAC56450.086.000945
GTATAGT100850.085.9083566
CCTATAC22800.085.837815
TATAGCG62800.085.542817
GTTATAG110950.085.414555
TATACGG95150.085.412567
Produced by FastQC (version 0.11.5)