FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l02_n01_N_Light097.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l02_n01_N_Light097.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3581806
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGA150020.4188389879295529No Hit
CAAGGCTAAATACGGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGTA90720.2532800492265634No Hit
CGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAAG84130.23488150949548914No Hit
GCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAA66460.18554885440473326No Hit
GGGCGGTAAATTCCGTCCAAGGCTAAATACGGGCGAGAGACCGATAGCGA62770.17524678891039883No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG89900.091.722595
CTATAGG52800.091.130096
GTATAGG96400.090.169646
CTATACG41100.089.566616
CCTATAC24700.089.421565
CGTATAG52850.089.066625
GTATAGC45200.088.9033666
TATAGGG110150.088.615077
CGTATAC30850.088.223795
TTATACG70300.088.0390556
TTATAGC53800.088.0236056
TATAGGC68450.087.849737
GCTATAG54100.087.447695
GGTATAA82250.087.4331745
GGTATAC48050.087.3866655
AGTATAG61450.087.345965
GTATAGT90350.087.270256
GTATACG61450.086.7275856
CCTATAG29450.086.611445
GTTATAG99400.086.444715
Produced by FastQC (version 0.11.5)