FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l02_n01_N_Light096.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l02_n01_N_Light096.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4233459
Sequences flagged as poor quality0
Sequence length101
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAACGATGCCGACCAGGGATCAGCGGATGTTGCTTATAGGACT46410.10962666698791698No Hit
GTTGCTTATAGGACTCCGCTGGCACCTTATGAGAAATCAAAGTTTTTGGG44160.10431186412812785No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG77500.091.261355
CGTATAG53150.089.727245
TATACGG53000.089.442397
CGTATAC36300.089.373345
GTATAGG90050.089.249426
GTATACG46050.089.222676
GTATAGC47150.089.055216
TATAGCG64850.088.699857
GTATACC40450.088.070716
TATAGGC55150.087.9364557
GCTATAC42650.087.872275
GCTATAG55700.087.75145
CTATACG35550.087.382996
TTATACG53700.087.30356
CTATAGG54150.086.753426
CCTATAG34500.086.326145
GTTATAG95550.085.952445
GGTATAC48800.085.558465
TTATAGC61950.085.491376
CGCGATA2950.085.3783652
Produced by FastQC (version 0.11.5)