FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l02_n01_N_Light095.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l02_n01_N_Light095.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9660111
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGCTTATAGGACTCCGCTGGCACCTTATGAGAAATCAAAGTTTTTGGG220790.22855845031180283No Hit
CAACCATAAACGATGCCGACCAGGGATCAGCGGATGTTGCTTATAGGACT170770.1767785069964517No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG167300.091.5090265
CGTATAC93350.091.08325
CTATACG95950.090.69156
GTATACG107950.090.5542456
GTATAGC115600.090.478536
CTATAGG131300.090.295466
GGTATAC105900.090.29165
GCTATAC110150.090.171465
GTATAGG196450.089.896646
GCTATAG135350.089.5967255
TTATACG130350.089.1686
TATACGG121000.089.10917
CCTATAG76200.089.016975
TATAGGC126650.088.959247
CCTATAC82200.088.5869755
GTATACC96400.088.494326
CGTATAG128750.088.4711155
TTATAGC145350.087.808946
GTTATAG212600.087.695235
TATACGC96500.087.4172747