FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l02_n01_N_Light094.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l02_n01_N_Light094.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5060828
Sequences flagged as poor quality0
Sequence length101
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG95650.090.978365
CGTATAG54150.088.334155
GTATAGG99600.088.3214956
TATACGG57700.088.000817
GTATACG51700.087.746
GCTATAC44750.087.039925
CCTATAG34950.086.982215
GTATAGC53900.086.80256
CTATACG38350.086.575816
CGTATAC42350.086.364445
CTATAGG59400.085.722166
GTATACC42300.085.341066
TATAGCG62450.085.262487
GTATAGT111700.085.004986
TATACTG89050.084.6501547
GCTATAG63400.084.1372155
TATAGGC59750.084.027587
CCTATAC33750.083.882265
GTTATAG117200.083.733715
GGTATAC54300.083.541295
Produced by FastQC (version 0.11.5)