FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l02_n01_N_Light093.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l02_n01_N_Light093.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4848109
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAACGATGCCGACCAGGGATCAGCGGATGTTGCTTATAGGACT76480.15775222875558284No Hit
GTTGCTTATAGGACTCCGCTGGCACCTTATGAGAAATCAAAGTTTTTGGG54920.11328128142333432No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG96250.091.248255
GTATACG55850.090.3202746
GTATAGC60150.089.943966
CGTATAC47250.089.8719255
TATACGG65850.089.732247
CTATACG46800.089.7202156
GCTATAC55400.089.59735
GTATACC49800.089.1797266
CTATAGG64350.088.871366
GTATAGG104250.088.6191256
CCTATAG39500.088.3855
GCGCCTA2800.088.252362
TATAGCG80650.088.224947
GGTATAC59200.088.178835
CCTATAC39100.088.074395
TATAGGC67600.087.339027
CGTATAG64350.086.8792955
GTTATAG112200.086.404935
TTATAGC76250.086.276756
TATAACG59100.086.2374957
Produced by FastQC (version 0.11.5)