FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l02_n01_N_Light092.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l02_n01_N_Light092.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6015730
Sequences flagged as poor quality0
Sequence length101
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAAG136480.22687188421022883No Hit
GCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAA93220.15496041211955988No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG121450.090.620575
GTATAGG127100.090.326446
CGTATAG66950.089.9636155
CGTATAC45000.089.9342965
CTATACG51000.089.875426
GTATACG64900.089.5819556
GCTATAC53450.089.2245255
GTATAGC68100.089.1389546
GGTATAC64550.088.157415
TATAGGC83400.087.195277
GTTATAG136100.087.183145
TTATACG79850.086.9673546
TATACTG96450.086.872077
GCTATAG72150.086.244865
TATACGG82250.085.931197
TTATAGC97300.085.820266
TATACGC58950.085.3288657
GTATAGT128100.085.320086
TATAACG55650.085.267627
AGTATAG84350.085.089825