FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l02_n01_N_Light091.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l02_n01_N_Light091.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8024694
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAACGATGCCGACCAGGGATCAGCGGATGTTGCTTATAGGACT116680.14540118289868748No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG156000.091.378165
GCTATAC85250.089.763995
GTATACG89400.089.6849756
CGTATAC71100.089.456525
CTATACG76000.089.2481846
GTATACC79400.088.7765356
GTATAGC95650.088.741016
GTATAGG178650.088.218196
TATACGG111600.087.93287
CTATAGG107150.087.37346
CGTATAG106750.087.303595
TATAGGC116050.086.6893167
GGTATAC98850.086.496165
TATAGCG126800.086.2324757
AGTATAC78100.086.1826555
CCTATAG62050.085.968425
GCTATAG115050.085.835895
TTATACG109050.085.6332866
TATAACG93600.085.305437
ATACGGC21200.084.9157268
Produced by FastQC (version 0.11.5)