FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l02_n01_N_Light090.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l02_n01_N_Light090.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4057359
Sequences flagged as poor quality0
Sequence length101
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAACGATGCCGACCAGGGATCAGCGGATGTTGCTTATAGGACT48650.11990558390322374No Hit
GTTGCTTATAGGACTCCGCTGGCACCTTATGAGAAATCAAAGTTTTTGGG43920.10824775426576745No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG73200.091.171915
TATACGG48550.089.421557
CGCGCTA2950.088.613282
GTATACG41800.088.293746
GTATAGG84450.088.136246
CGTATAC33150.088.1226355
GTATACC33850.087.982046
GCTATAC40800.087.898745
CGTATAG49250.087.670515
CTATACG35850.087.31356
TATAGGC54250.087.030577
GTATAGC47950.086.77626
CCTATAC27700.086.7693945
CCTATAG32800.086.745875
GCTATAG55350.085.646345
CTATAGG52050.084.868666
TATAGCG62100.084.825247
GTTATAG89600.084.715845
GGTATAC45700.084.2947545
TTATACG50150.084.106046
Produced by FastQC (version 0.11.5)