FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l02_n01_N_Light089.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l02_n01_N_Light089.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4808169
Sequences flagged as poor quality0
Sequence length101
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG90450.089.960075
GCTATAC54000.088.3163155
TATACGG60050.088.274267
GTATAGG99850.088.195126
CGTATAG57050.088.090895
CTATACG43350.086.889446
GTATAGC57500.086.654396
CGTATAC41500.086.531595
TATAGGC60250.086.0891657
CTATAGG58200.085.693766
TATAGCG58500.085.4166957
GTATACG50900.084.919326
CCTATAG38700.083.954915
GTATACC41100.083.9030466
GGTATAC58900.083.549815
CCTATAC37200.083.509485
TTATACG59700.083.46116
TATAGGG105400.083.325757
GTTATAG120000.082.888915
GCTATAG67950.082.838045
Produced by FastQC (version 0.11.5)