FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l02_n01_N_Light088.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l02_n01_N_Light088.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4278729
Sequences flagged as poor quality0
Sequence length101
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG80700.090.000285
GTATAGG81650.089.5878456
GCTATAC40150.089.561275
CGTATAG45400.088.411915
GTATACG37750.088.329286
CGTATAC34100.087.341995
GTATAGC45150.086.7922066
CCTATAG28750.086.081495
GCTATAG54100.085.784185
TATACTG69300.085.606887
TATAGCG52500.085.587687
TATACGG48450.085.389377
TATAGGC52150.085.342387
CTATACG30950.085.022466
GTTATAG92900.084.7265
GTATACC30950.084.562056
CCTATAC28200.084.3914645
AGTATAG61100.084.274855
TTATACG49350.084.2180946
CCGTATA36650.084.1163944
Produced by FastQC (version 0.11.5)