FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l02_n01_N_Light087.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l02_n01_N_Light087.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6312261
Sequences flagged as poor quality0
Sequence length101
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG110950.089.822345
GTATAGG119400.088.39526
GTATAGC69250.088.208446
GTATACG61950.088.175016
CGTATAC53450.087.89315
TATACGG73050.087.6506657
CTATACG50750.087.5113756
GCTATAC61400.087.498465
CGTATAG74900.087.265545
TATAGCG83050.087.0483557
CCTATAG46600.086.644145
TATAGGC77500.086.11137
CTATAGG76000.086.061586
GTATACC50450.085.018926
TATAACG66400.084.196487
AGTATAC55200.084.073995
CCTATAC47700.084.048555
GGTATAC68050.083.764365
GTTATAG138950.083.721385
GCTATAG86650.083.3261265
Produced by FastQC (version 0.11.5)