FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l02_n01_N_Light086.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l02_n01_N_Light086.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6212142
Sequences flagged as poor quality0
Sequence length101
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAACGATGCCGACCAGGGATCAGCGGATGTTGCTTATAGGACT70810.11398644783071604No Hit
GTTGCTTATAGGACTCCGCTGGCACCTTATGAGAAATCAAAGTTTTTGGG66120.1064367169971324No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG106550.090.6319055
GTATACG65600.089.42246
GTATAGG121100.089.1142966
GTATACC55650.088.937676
CGTATAC54300.088.527435
TATACGG79350.087.934257
CTATACG54550.087.857666
GCTATAC66150.087.820075
GTATAGC69450.087.3377156
CGTATAG72900.087.3120965
CCTATAG46200.087.186865
CTATAGG78900.086.810346
TATAGCG92950.086.514877
GGTATAC70400.086.499295
TATAGGC80800.086.297437
GCTATAG83450.085.495015
TTATACG78700.084.918546
GTTATAG130950.084.91675
AGTATAC55850.084.709745
CCGTATA54650.084.049194
Produced by FastQC (version 0.11.5)