FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l02_n01_N_Light085.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l02_n01_N_Light085.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7343450
Sequences flagged as poor quality0
Sequence length101
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAACGATGCCGACCAGGGATCAGCGGATGTTGCTTATAGGACT90720.12353866370711315No Hit
GTTGCTTATAGGACTCCGCTGGCACCTTATGAGAAATCAAAGTTTTTGGG90490.12322545942302324No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG130100.091.459095
GTATAGC85500.090.109256
CGTATAC69100.089.9825065
CGTATAG89750.089.866885
GTATAGG143100.089.819956
CTATAGG90650.089.339036
TATACGG90600.088.811637
GGTATAC83950.088.60695
GCTATAC81650.088.6013265
GTATACG82100.088.4025656
CCTATAC59200.088.260725
CTATACG68500.088.202566
CCTATAG54700.088.0536045
GTATACC68750.087.812746
TATAGCG114400.086.943147
TTATACG97250.086.646026
GCTATAG104850.086.3477255
GTTATAG160850.086.289045
TATAGCC62000.085.95797
GATATAG115200.085.888155
Produced by FastQC (version 0.11.5)