FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l02_n01_N_Light084.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l02_n01_N_Light084.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5160801
Sequences flagged as poor quality0
Sequence length101
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG99350.090.5551455
GTATACG51850.090.3259056
CTATACG44900.089.708526
GTATAGG109300.089.131186
GCTATAC55400.088.6567465
TATAGCG66700.088.375147
CGTATAG59550.087.902425
TATACGG62650.087.87127
GTATAGC59700.087.59866
CCTATAG38150.087.032935
CGTATAC43350.086.23565
TATAGGC68400.085.8314747
GTATACC47950.085.0919956
GATATAG83900.084.357815
GCTATAG75400.084.291885
CTATAGG68700.084.281296
TATACTG82700.084.142677
GTTATAG116050.083.868295
TTATACG64400.083.860766
TATAACG52400.083.485787
Produced by FastQC (version 0.11.5)