FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l02_n01_N_Light083.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l02_n01_N_Light083.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4662713
Sequences flagged as poor quality0
Sequence length101
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACG46800.090.025776
TATACGG53350.089.5669867
CGTATAC40050.089.188545
GTATAGG93550.089.0583956
CGTATAG52600.089.039945
CTATACG38100.089.014826
GGTATAG85400.088.9935
GCTATAC46600.088.068695
GTATAGC48150.087.107086
TATAGCG59500.086.615817
GTATACC44050.086.372536
TATAGGC59750.086.332897
CCTATAG37600.086.156945
TTATACG55100.085.688756
AGTATAC44750.085.553095
GGTATAC54750.084.762585
TATACTG74650.084.753727
CCTATAC38600.084.4171145
GTTATAG100450.083.698385
TATAGCC38550.083.662297
Produced by FastQC (version 0.11.5)