FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l02_n01_N_Light049.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l02_n01_N_Light049.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8318558
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAAG140830.16929616887926968No Hit
GTTGCTTATAGGACTCCGCTGGCACCTTATGAGAAATCAAAGTTTTTGGG115130.13840139120265796No Hit
GCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAA94870.11404620848950023No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACG91900.092.2585456
GGTATAG156750.092.242035
CCTATAC70250.092.227365
CTATACG78850.092.106376
GTATAGG186200.092.0132456
GCTATAC94550.091.834615
GTATAGC106400.091.828296
TTATAGC138800.091.781086
GCTATAG117200.091.5546345
CGTATAG117400.091.317745
GGTATAC96850.091.125055
TTATACG107900.090.639776
CTATAGC96950.090.6374746
CCTATAG70950.090.31325
AGTATAG116750.090.280125
CGTATAC87450.090.274055
TATACGC93400.089.9631967
GATATAG133050.089.858685
TTATACC102250.089.795056
GTATACC78650.089.622916