FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l02_n01_N_Light048.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l02_n01_N_Light048.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences282574
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCACACGTCTGAACTCCAGTCACTCGAGGATCTCGTATGCCGT3130.11076744498786158TruSeq Adapter, Index 10 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGTA409.094947E-1295.0618062
TGGAGGT201.735928E-595.0618061
GTCGCTA201.735928E-595.0618062
GGTACTA156.595641E-495.0618062
TCGCATA301.2230885E-895.0618062
CACGGTA156.595641E-495.0618062
GCGAATT201.735928E-595.0618061
CCGATGT201.735928E-595.0618061
GAGTCGT156.595641E-495.0618061
GAGTACT156.595641E-495.0618061
GGCCGTA201.735928E-595.0618062
GCCGGTA156.595641E-495.0618062
GCGCCTA156.595641E-495.0618062
CCTACCT201.735928E-595.0618061
CCGCTTA201.735928E-595.0618062
GCCATTA156.595641E-495.0618062
CGACAAT201.735928E-595.0618061
GACCCAT156.595641E-495.0618061
CAGGGTA156.595641E-495.0618062
GCACATA550.095.0618062
Produced by FastQC (version 0.11.5)