FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l02_n01_N_Light047.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l02_n01_N_Light047.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7853638
Sequences flagged as poor quality0
Sequence length101
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG151300.090.634825
GTATAGG155050.089.605736
GCTATAC83450.089.0220645
CGTATAG84850.088.672835
CTATACG62850.088.195686
TATACGG86100.088.046377
GTATAGC83700.087.4498146
CCTATAC55800.086.826685
CGTATAC66400.086.700535
GTATACG79200.086.661136
CCTATAG59450.085.9701465
GTATACC64300.085.172626
TATAGCG85500.085.053227
TATAGGC97650.084.442137
CTATAGG96600.084.425746
AGTATAC70350.084.128115
GCGCTAT27550.083.964283
GCTATAG106800.083.746435
CCGTATA66650.083.310854
TATACTG138050.083.299067
Produced by FastQC (version 0.11.5)