FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l02_n01_N_Light046.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l02_n01_N_Light046.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6259505
Sequences flagged as poor quality0
Sequence length101
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG117050.089.404435
GTATAGG119300.089.304546
GCTATAC66850.088.325425
GTATACG61300.087.869336
TATACGG71550.087.762017
CTATACG52850.087.6282966
CGTATAG71550.087.436365
TATAGGC74200.086.676137
CGTATAC50600.086.368085
CCTATAC46950.085.4942865
GTATAGC70500.085.094016
GTATACC50200.084.4006656
TATACTG105100.084.377447
GCTATAG84700.084.2935
CCTATAG47600.084.1272355
TATAGCG68650.083.9968267
CTATAGG76550.083.5809256
TTATACG73550.083.438136
CGCGCTA5200.083.153862
TATACGC52350.082.930497
Produced by FastQC (version 0.11.5)