FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l02_n01_N_Light045.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l02_n01_N_Light045.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4437906
Sequences flagged as poor quality0
Sequence length101
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG78700.090.355495
GTATAGG80650.089.2269746
CGTATAG49700.088.121535
GCTATAC47600.087.518555
CTATACG33850.087.140656
CCTATAC33800.087.132965
GTATACG38900.086.45136
GTATAGC48150.085.9230656
CGTATAC35800.085.847715
TATAGCG50150.085.811437
TATACGG49600.085.805327
CCTATAG33100.085.3879245
TATAGGC49400.084.998887
GTATACC32750.084.41116
GCTATAG59100.083.750565
TTATACG54200.083.693546
AGTATAC36450.083.143945
GTATAGT94100.082.429846
TATACTG78200.082.30397
CCGTATA37350.082.285094
Produced by FastQC (version 0.11.5)