FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l02_n01_N_Light044.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l02_n01_N_Light044.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4697946
Sequences flagged as poor quality0
Sequence length101
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG87900.090.8930745
GTATAGG84300.089.870586
CGTATAG49400.089.230785
GCTATAC50750.089.197055
TATACGG54500.088.722897
GTATACG43550.087.581386
CTATACG38400.087.452576
CGTATAC40000.087.0437555
CCTATAG34800.086.400865
GTATAGC51250.086.285976
TATAGGC59650.086.159117
GTATACC39250.086.042776
GCTATAG64200.085.307645
CCTATAC36650.085.020485
ATACGGC12150.084.442658
TTATACG57750.084.305576
CCGCGTA2200.084.2442
TATAGCG56500.084.153087
TATACTG77550.083.850587
CGCGCTA3800.083.7892462
Produced by FastQC (version 0.11.5)