FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l02_n01_N_Light043.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l02_n01_N_Light043.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4214342
Sequences flagged as poor quality0
Sequence length101
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG84300.089.983346
GCTATAC46050.089.948155
GGTATAG82100.089.8532265
CTATACG37900.089.4836656
TATACGG53000.089.35097
CGTATAG47800.088.841325
CGTATAC38750.087.647545
CGCGCTA3850.087.642412
CCTATAC35350.087.343255
CCTATAG31650.086.8980945
GTATACG44350.086.7513356
TATAGCG55450.086.173967
GTATACC35900.086.001036
TATAGGC54750.085.627377
TATACTG72050.085.306077
GTATAGC50150.085.147816
AGTATAC37450.084.982285
TATAACG41650.084.505087
TATAGCC37700.084.413737
GTTATAG92450.083.7502445
Produced by FastQC (version 0.11.5)