FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l02_n01_N_Light042.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l02_n01_N_Light042.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7609648
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGCTTATAGGACTCCGCTGGCACCTTATGAGAAATCAAAGTTTTTGGG115690.15203068525640082No Hit
CAACCATAAACGATGCCGACCAGGGATCAGCGGATGTTGCTTATAGGACT114500.1504668809910787No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTATAC65650.092.4001165
GGTATAG143500.092.125035
CTATACG84050.091.43846
GCTATAC88600.091.198345
CGTATAC76450.090.779765
GCTATAG114800.090.5360955
GTATACG83250.090.4342356
GTATAGG186650.089.807276
TTATAGC123500.089.2680366
GGTATAC95500.089.135555
TTATACG108900.089.0667656
TATACGG117000.088.867897
CCTATAG74300.088.867375
GTATACC81600.088.712056
TTATACC111200.088.676876
AGTATAG117150.088.476685
CTATAAC97850.088.154176
GATATAC90800.088.151645
TATAGGC129050.088.004847
TCTATAG109200.087.957895
Produced by FastQC (version 0.11.5)