FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l02_n01_N_Light041.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l02_n01_N_Light041.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7187739
Sequences flagged as poor quality0
Sequence length101
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG126100.090.366865
GTATAGG130650.090.0536966
CGTATAG78500.089.856775
TATACGG81700.087.730817
GCTATAC75550.087.70695
CGTATAC59300.087.230275
GTATAGC74450.087.150726
CTATACG60800.086.170166
GTATACG68650.086.072586
GCTATAG92450.085.9059755
TATAGGC83350.085.02537
CCTATAC54100.084.903045
CCTATAG54250.084.0553745
GTATACC54550.083.939676
TATAGGT154200.083.9396447
CTATAGG83050.083.845416
CCGTATA63650.083.65684
GCGCTAT22900.083.591783
TATACTG132150.082.993057
GCGCCTA6250.082.8689042
Produced by FastQC (version 0.11.5)