FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l02_n01_N_Light040.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l02_n01_N_Light040.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5499313
Sequences flagged as poor quality0
Sequence length101
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG100100.090.776865
GTATAGG101750.089.582466
CGTATAG60600.088.259215
CGTATAC47200.088.257555
TATACGG59200.088.177637
GCTATAC55950.087.5291755
GTATAGC59750.086.2530066
CTATACG46550.086.0181966
TATAGCG59050.085.9080357
TATAGGC62350.085.4749457
CCTATAG40350.085.464815
GCTATAG72550.085.375735
GTATACG51250.085.173426
CCTATAC41250.085.09715
GTATACC43550.084.85436
GCGCTAT18750.084.6134643
TATACTG98700.084.169577
CTATAGG68800.083.951336
CCGTATA50000.083.885134
TATAGCC44350.083.537887
Produced by FastQC (version 0.11.5)