FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l02_n01_N_Light039.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l02_n01_N_Light039.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2717259
Sequences flagged as poor quality0
Sequence length101
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG48050.090.153345
GTATAGG50400.089.907986
TATACGG32300.088.379667
GCTATAC27950.088.1994555
GTATAGC27300.088.037616
CGTATAG28000.087.702695
TATAGCG30950.087.6306157
CCTATAC18700.087.377025
CGTATAC23000.087.149525
CTATACG22200.086.866736
GCTATAG34950.085.483815
CCTATAG17650.085.309025
CCGCGTA1400.084.85322
GTATACG25900.084.727156
GTTATAG60500.083.770185
GTATACC22850.083.564196
TATAGGC31650.083.4413767
TATACTG47450.083.1850367
TATAGGT59850.082.933987
TTATACG33400.082.909166
Produced by FastQC (version 0.11.5)