FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l02_n01_N_Light038.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l02_n01_N_Light038.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3344004
Sequences flagged as poor quality0
Sequence length101
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCATTTATTGCTACTGCACTCTTCATTCTCGTTCCTACTGCTTTTTTGC40640.12153095510651303No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG67400.090.133936
GGTATAG64950.089.951865
CTATACG27150.089.223256
CGTATAG37450.088.529455
GCTATAC32500.088.421155
CCTATAC22250.088.380115
GTATAGC36650.088.257336
TATACGG37600.087.669667
CTATAGG41000.087.118766
GCGCTAT8800.086.361763
CGTATAC27450.086.346035
GTATACC23200.085.988236
TATAGGC40200.085.898597
GTATACG33700.085.694186
ATACGGC7900.085.376628
CCTATAG23050.085.312685
TATAGCG36600.085.133497
GCTATAG43850.084.1657945
TCTATAG47050.083.993935
TTATACG38500.083.1527946
Produced by FastQC (version 0.11.5)