FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l02_n01_N_Light037.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l02_n01_N_Light037.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3026585
Sequences flagged as poor quality0
Sequence length101
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG52900.089.794615
GCTATAC28300.089.463665
CTATACG23450.089.123916
CGTATAG32550.088.873485
TATACGG32950.088.511027
GTATAGG56550.088.44646
CCGCGTA1400.088.251782
CGTATAC23900.088.245185
CCTATAC22250.088.1710365
GTATACG27700.087.62456
GTATACC25500.085.870736
CCTATAG20950.085.706495
GCTATAG35850.085.595175
AGTATAG42650.085.5358355
ATACGGC6750.085.146358
GTATAGT63150.084.84386
TATAGGC34550.084.274627
TATACTG51150.083.947387
GCGCTAT9750.083.797253
GGTATAC34200.083.752375
Produced by FastQC (version 0.11.5)