FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l02_n01_N_Light036.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l02_n01_N_Light036.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5369840
Sequences flagged as poor quality0
Sequence length101
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG97350.089.24295
GTATAGG100600.089.1911546
CGTATAC43600.088.899555
CGTATAG58550.088.185785
GCTATAC53250.088.131925
GTATAGC56500.088.105026
CTATACG42500.088.069416
TATACGG59900.087.386157
GCGCTAT16300.086.258123
GTATACG52600.085.968446
TATAGGC63400.085.93347
CGCGCTA4050.085.644852
CCTATAG41800.085.114095
GTATACC41400.084.787516
CCTATAC39950.084.775175
TATACTG92350.084.6090557
TATAGCG63600.084.542897
TTATACG63950.084.005926
AGTATAG76950.083.951065
TATAACG48900.083.633857
Produced by FastQC (version 0.11.5)