FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l02_n01_N_Light035.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l02_n01_N_Light035.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6815696
Sequences flagged as poor quality0
Sequence length101
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG132450.091.163875
GTATAGG137800.089.586036
CGTATAG78450.089.006875
CTATACG59100.088.9699556
GCTATAC72850.088.350475
CGTATAC61900.088.018085
TATACGG77750.087.422357
GTATAGC78300.086.26236
GTATACG69450.086.175046
CCTATAG51850.085.748445
TATAGCG82450.085.665027
GTATACC57850.085.637546
GCTATAG94150.085.617135
TATAGGC87800.085.368117
GCGCTAT23400.085.05453
AGTATAG101750.084.637455
TATAACG68400.084.511877
CGCGCTA5300.084.290262
GTATAGT146150.084.07776
TATACTG118700.083.913327
Produced by FastQC (version 0.11.5)