FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l02_n01_N_Light034.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l02_n01_N_Light034.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4297140
Sequences flagged as poor quality0
Sequence length101
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG79100.090.8566365
GTATAGG78400.089.4209446
CGTATAG45250.088.8067555
GCTATAC42400.088.61455
TATACGG49200.088.2368557
CTATACG33350.088.158416
CGTATAC37100.086.934085
GTATAGC49500.086.262776
GTATACG41400.086.045846
CCTATAC32350.086.043395
CCTATAG32100.085.529715
GCTATAG57200.085.035085
TATAGCG47000.084.8887947
TATAGGC49750.084.874577
GCGCTAT13600.084.872433
GTATACC33300.084.297016
TTATACG51700.083.9700856
CGCGCTA3350.083.708462
TATACTG72850.083.519627
GTTATAG95350.083.4426965
Produced by FastQC (version 0.11.5)