FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l02_n01_N_Light033.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l02_n01_N_Light033.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2496059
Sequences flagged as poor quality0
Sequence length101
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG45450.090.817035
GTATAGG48200.090.661366
GCTATAC26500.090.1578455
CGTATAG28950.089.582985
CTATACG20350.088.92876
TACGCGC750.088.664189
CCTATAG18350.088.008445
CGTATAC21800.087.153525
GTATACG24500.087.048586
TATAGGC30350.086.7026757
GTATAGC28750.086.571496
TATAGCG30250.086.361227
TATACGG30900.085.313137
TTATACG32150.085.098716
TATACGC22050.085.088327
GCTATAG34050.084.8140645
GTTATAG52200.084.5330665
TATACTG43200.084.112237
GTATACC20400.084.054026
TATAACG23400.084.03617
Produced by FastQC (version 0.11.5)