FastQCFastQC Report
Sat 29 Feb 2020
HKMLVDRXX_l01_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKMLVDRXX_l01_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105787478
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGTGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7513730.710266483524638No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGGGG1257900.086.747077
GGGGTGT1274550.086.7016143
GTGTGGG1265650.086.542236
GGGTGTG1281750.086.099774
GTATAGC61600.080.3822256
TATACGG66050.080.3573157
GGTATAC68250.078.880035
GCTATAC60900.078.188035
CGTATAG73050.077.791225
GTATACG60400.077.7348566
TATACTG100900.077.5864647
GTATAGG120700.076.619296
GGTATAG111950.075.9500665
CCGTATA55850.075.397594
TATAAGG105250.075.372137
CTATAGG71200.074.2782446
GTATAGT130800.073.9332356
CTATACG53750.073.927066
CGTATAC56000.072.991425
TTATACG79650.072.83536
Produced by FastQC (version 0.11.5)